Experiment 1: Ubiquity of Microorganisms
Procedure
Terms & Definitions
Culture media (medium, singular): solution of nutrients required for the growth of bacteria.
Agar: a carbohydrate derived from seaweed used to solidify a liquid medium.
Tryptic Soy Agar (TSA): a rich solidmedium containing a digest of casein (the principal milk protein) and soy products. It is an all-purpose medium that supports the growth of many diverse organisms.
Tryptic Soy Broth (TSB): a rich liquidmedium containing a digest of casein and soy products. It is a general-purpose medium that supports the growth of organisms that are not exacting in their food requirements.
Colony: a visible population of microorganisms originating from a single parent cell and growing on a solid medium.
Agar: a carbohydrate derived from seaweed used to solidify a liquid medium.
Tryptic Soy Agar (TSA): a rich solidmedium containing a digest of casein (the principal milk protein) and soy products. It is an all-purpose medium that supports the growth of many diverse organisms.
Tryptic Soy Broth (TSB): a rich liquidmedium containing a digest of casein and soy products. It is a general-purpose medium that supports the growth of organisms that are not exacting in their food requirements.
Colony: a visible population of microorganisms originating from a single parent cell and growing on a solid medium.
Results & Examples
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Nutrient Broth Tube
Also observe the cloudiness (turbidity) of your nutrient broth tube and estimate the number of bacteria per mL. |